Recombinant:Article Title: AstA Signaling Functions as an Evolutionary Conserved Mechanism Timing Juvenile to Adult Transition.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig monoclonal anti-PTTH [28] N/A Rat monoclonal anti-dilp2 [16] N/A Mouse monoclonal anti-AstA DSHB 5F10 Rabbit polyclonal anti-AstA Jena Bioscience ABD-062 Chicken monoclonal anti-GFP Sigma G6539 Alexa Fluor 488 polyclonal anti-chicken Thermo Fisher Scientific A11039 Alexa Fluor 488 polyclonal anti-mouse Thermo Fisher Scientific A11029 Alexa Fluor 546 polyclonal anti-rat Thermo Fisher Scientific A11081 Alexa Fluor 647 polyclonal anti-rabbit Thermo Fisher Scientific A31573 Mouse monoclonal anti-FLAG Sigma-Aldrich F1804 Chemicals, Peptides, and Recombinant Proteins Vectashield Mounting Medium Vector laboratories H-1200 Formaldehyde, 16% Polysciences 18814-10 RNeasy lipid tissue Minikit QIAGEN 74804 SuperScript II reverse transcriptase Invitrogen 18064014 Power SYBR Green PCR mastermix Applied Biosystems 4367659 F8 Maxisorp loose Nunc-Immuno modules Thermo Scientific 469949 BuPH Carbonate-Bicarbonate buffer packs ThermoFisher 29892 anti-HA-Peroxidase, High Affinity (3F10) Roche 12013819001 1-Step Ultra TMB- ELISA Substrate Thermo Scientific 34029 Critical Commercial Assays Spi Bio 20-HE ELISA kit Spi Bio A05120 20-HE Sigma H5142 Experimental Models: Organisms/Strains Drosophila NP423-Gal4 DGRC 103614 Drosophila ptth-Gal4 [13] N/A Drosophila Dilp2-Gal4 [51, 52] N/A Drosophila Tsh-Gal80 [53] N/A Drosophila AstA-Gal4 BDSC 39351 Drosophila ptth-HA genomic [54] N/A Drosophila UAS-TRPA1 [55] N/A Drosophila Ilp2HF [56] N/A Drosophila Dilp2-LexA [57] N/A Drosophila AstAR1-Gal4 [21] N/A Drosophila AstA-RNAi VDRC 14398 Drosophila AstAR1-RNAi VDRC 39222 Drosophila AstAR1-RNAi VDRC 48495 Drosophila ptth-RNAi VDRC 102043 Drosophila UAS-dicer2 VDRC 60008 Drosophila UAS-dicer2 VDRC 60009 Drosophila op-LexA-GFP BDSC 58754 Drosophila UAS-RFP BDSC 58754 Drosophila CoinFLP-LexA::GAD BDSC 58753 Drosophila UAS-Kir2.1 BDSC 6595 (Continued on next page) Current Biology 29, 1–10.e1–e4, March 4, 2019 e1 ..
Reverse Transcription:Article Title: AstA Signaling Functions as an Evolutionary Conserved Mechanism Timing Juvenile to Adult Transition.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig monoclonal anti-PTTH [28] N/A Rat monoclonal anti-dilp2 [16] N/A Mouse monoclonal anti-AstA DSHB 5F10 Rabbit polyclonal anti-AstA Jena Bioscience ABD-062 Chicken monoclonal anti-GFP Sigma G6539 Alexa Fluor 488 polyclonal anti-chicken Thermo Fisher Scientific A11039 Alexa Fluor 488 polyclonal anti-mouse Thermo Fisher Scientific A11029 Alexa Fluor 546 polyclonal anti-rat Thermo Fisher Scientific A11081 Alexa Fluor 647 polyclonal anti-rabbit Thermo Fisher Scientific A31573 Mouse monoclonal anti-FLAG Sigma-Aldrich F1804 Chemicals, Peptides, and Recombinant Proteins Vectashield Mounting Medium Vector laboratories H-1200 Formaldehyde, 16% Polysciences 18814-10 RNeasy lipid tissue Minikit QIAGEN 74804 SuperScript II reverse transcriptase Invitrogen 18064014 Power SYBR Green PCR mastermix Applied Biosystems 4367659 F8 Maxisorp loose Nunc-Immuno modules Thermo Scientific 469949 BuPH Carbonate-Bicarbonate buffer packs ThermoFisher 29892 anti-HA-Peroxidase, High Affinity (3F10) Roche 12013819001 1-Step Ultra TMB- ELISA Substrate Thermo Scientific 34029 Critical Commercial Assays Spi Bio 20-HE ELISA kit Spi Bio A05120 20-HE Sigma H5142 Experimental Models: Organisms/Strains Drosophila NP423-Gal4 DGRC 103614 Drosophila ptth-Gal4 [13] N/A Drosophila Dilp2-Gal4 [51, 52] N/A Drosophila Tsh-Gal80 [53] N/A Drosophila AstA-Gal4 BDSC 39351 Drosophila ptth-HA genomic [54] N/A Drosophila UAS-TRPA1 [55] N/A Drosophila Ilp2HF [56] N/A Drosophila Dilp2-LexA [57] N/A Drosophila AstAR1-Gal4 [21] N/A Drosophila AstA-RNAi VDRC 14398 Drosophila AstAR1-RNAi VDRC 39222 Drosophila AstAR1-RNAi VDRC 48495 Drosophila ptth-RNAi VDRC 102043 Drosophila UAS-dicer2 VDRC 60008 Drosophila UAS-dicer2 VDRC 60009 Drosophila op-LexA-GFP BDSC 58754 Drosophila UAS-RFP BDSC 58754 Drosophila CoinFLP-LexA::GAD BDSC 58753 Drosophila UAS-Kir2.1 BDSC 6595 (Continued on next page) Current Biology 29, 1–10.e1–e4, March 4, 2019 e1 ..
SYBR Green Assay:Article Title: AstA Signaling Functions as an Evolutionary Conserved Mechanism Timing Juvenile to Adult Transition.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig monoclonal anti-PTTH [28] N/A Rat monoclonal anti-dilp2 [16] N/A Mouse monoclonal anti-AstA DSHB 5F10 Rabbit polyclonal anti-AstA Jena Bioscience ABD-062 Chicken monoclonal anti-GFP Sigma G6539 Alexa Fluor 488 polyclonal anti-chicken Thermo Fisher Scientific A11039 Alexa Fluor 488 polyclonal anti-mouse Thermo Fisher Scientific A11029 Alexa Fluor 546 polyclonal anti-rat Thermo Fisher Scientific A11081 Alexa Fluor 647 polyclonal anti-rabbit Thermo Fisher Scientific A31573 Mouse monoclonal anti-FLAG Sigma-Aldrich F1804 Chemicals, Peptides, and Recombinant Proteins Vectashield Mounting Medium Vector laboratories H-1200 Formaldehyde, 16% Polysciences 18814-10 RNeasy lipid tissue Minikit QIAGEN 74804 SuperScript II reverse transcriptase Invitrogen 18064014 Power SYBR Green PCR mastermix Applied Biosystems 4367659 F8 Maxisorp loose Nunc-Immuno modules Thermo Scientific 469949 BuPH Carbonate-Bicarbonate buffer packs ThermoFisher 29892 anti-HA-Peroxidase, High Affinity (3F10) Roche 12013819001 1-Step Ultra TMB- ELISA Substrate Thermo Scientific 34029 Critical Commercial Assays Spi Bio 20-HE ELISA kit Spi Bio A05120 20-HE Sigma H5142 Experimental Models: Organisms/Strains Drosophila NP423-Gal4 DGRC 103614 Drosophila ptth-Gal4 [13] N/A Drosophila Dilp2-Gal4 [51, 52] N/A Drosophila Tsh-Gal80 [53] N/A Drosophila AstA-Gal4 BDSC 39351 Drosophila ptth-HA genomic [54] N/A Drosophila UAS-TRPA1 [55] N/A Drosophila Ilp2HF [56] N/A Drosophila Dilp2-LexA [57] N/A Drosophila AstAR1-Gal4 [21] N/A Drosophila AstA-RNAi VDRC 14398 Drosophila AstAR1-RNAi VDRC 39222 Drosophila AstAR1-RNAi VDRC 48495 Drosophila ptth-RNAi VDRC 102043 Drosophila UAS-dicer2 VDRC 60008 Drosophila UAS-dicer2 VDRC 60009 Drosophila op-LexA-GFP BDSC 58754 Drosophila UAS-RFP BDSC 58754 Drosophila CoinFLP-LexA::GAD BDSC 58753 Drosophila UAS-Kir2.1 BDSC 6595 (Continued on next page) Current Biology 29, 1–10.e1–e4, March 4, 2019 e1 ..
Polymerase Chain Reaction:Article Title: AstA Signaling Functions as an Evolutionary Conserved Mechanism Timing Juvenile to Adult Transition.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig monoclonal anti-PTTH [28] N/A Rat monoclonal anti-dilp2 [16] N/A Mouse monoclonal anti-AstA DSHB 5F10 Rabbit polyclonal anti-AstA Jena Bioscience ABD-062 Chicken monoclonal anti-GFP Sigma G6539 Alexa Fluor 488 polyclonal anti-chicken Thermo Fisher Scientific A11039 Alexa Fluor 488 polyclonal anti-mouse Thermo Fisher Scientific A11029 Alexa Fluor 546 polyclonal anti-rat Thermo Fisher Scientific A11081 Alexa Fluor 647 polyclonal anti-rabbit Thermo Fisher Scientific A31573 Mouse monoclonal anti-FLAG Sigma-Aldrich F1804 Chemicals, Peptides, and Recombinant Proteins Vectashield Mounting Medium Vector laboratories H-1200 Formaldehyde, 16% Polysciences 18814-10 RNeasy lipid tissue Minikit QIAGEN 74804 SuperScript II reverse transcriptase Invitrogen 18064014 Power SYBR Green PCR mastermix Applied Biosystems 4367659 F8 Maxisorp loose Nunc-Immuno modules Thermo Scientific 469949 BuPH Carbonate-Bicarbonate buffer packs ThermoFisher 29892 anti-HA-Peroxidase, High Affinity (3F10) Roche 12013819001 1-Step Ultra TMB- ELISA Substrate Thermo Scientific 34029 Critical Commercial Assays Spi Bio 20-HE ELISA kit Spi Bio A05120 20-HE Sigma H5142 Experimental Models: Organisms/Strains Drosophila NP423-Gal4 DGRC 103614 Drosophila ptth-Gal4 [13] N/A Drosophila Dilp2-Gal4 [51, 52] N/A Drosophila Tsh-Gal80 [53] N/A Drosophila AstA-Gal4 BDSC 39351 Drosophila ptth-HA genomic [54] N/A Drosophila UAS-TRPA1 [55] N/A Drosophila Ilp2HF [56] N/A Drosophila Dilp2-LexA [57] N/A Drosophila AstAR1-Gal4 [21] N/A Drosophila AstA-RNAi VDRC 14398 Drosophila AstAR1-RNAi VDRC 39222 Drosophila AstAR1-RNAi VDRC 48495 Drosophila ptth-RNAi VDRC 102043 Drosophila UAS-dicer2 VDRC 60008 Drosophila UAS-dicer2 VDRC 60009 Drosophila op-LexA-GFP BDSC 58754 Drosophila UAS-RFP BDSC 58754 Drosophila CoinFLP-LexA::GAD BDSC 58753 Drosophila UAS-Kir2.1 BDSC 6595 (Continued on next page) Current Biology 29, 1–10.e1–e4, March 4, 2019 e1 ..
Enzyme-linked Immunosorbent Assay:Article Title: AstA Signaling Functions as an Evolutionary Conserved Mechanism Timing Juvenile to Adult Transition.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig monoclonal anti-PTTH [28] N/A Rat monoclonal anti-dilp2 [16] N/A Mouse monoclonal anti-AstA DSHB 5F10 Rabbit polyclonal anti-AstA Jena Bioscience ABD-062 Chicken monoclonal anti-GFP Sigma G6539 Alexa Fluor 488 polyclonal anti-chicken Thermo Fisher Scientific A11039 Alexa Fluor 488 polyclonal anti-mouse Thermo Fisher Scientific A11029 Alexa Fluor 546 polyclonal anti-rat Thermo Fisher Scientific A11081 Alexa Fluor 647 polyclonal anti-rabbit Thermo Fisher Scientific A31573 Mouse monoclonal anti-FLAG Sigma-Aldrich F1804 Chemicals, Peptides, and Recombinant Proteins Vectashield Mounting Medium Vector laboratories H-1200 Formaldehyde, 16% Polysciences 18814-10 RNeasy lipid tissue Minikit QIAGEN 74804 SuperScript II reverse transcriptase Invitrogen 18064014 Power SYBR Green PCR mastermix Applied Biosystems 4367659 F8 Maxisorp loose Nunc-Immuno modules Thermo Scientific 469949 BuPH Carbonate-Bicarbonate buffer packs ThermoFisher 29892 anti-HA-Peroxidase, High Affinity (3F10) Roche 12013819001 1-Step Ultra TMB- ELISA Substrate Thermo Scientific 34029 Critical Commercial Assays Spi Bio 20-HE ELISA kit Spi Bio A05120 20-HE Sigma H5142 Experimental Models: Organisms/Strains Drosophila NP423-Gal4 DGRC 103614 Drosophila ptth-Gal4 [13] N/A Drosophila Dilp2-Gal4 [51, 52] N/A Drosophila Tsh-Gal80 [53] N/A Drosophila AstA-Gal4 BDSC 39351 Drosophila ptth-HA genomic [54] N/A Drosophila UAS-TRPA1 [55] N/A Drosophila Ilp2HF [56] N/A Drosophila Dilp2-LexA [57] N/A Drosophila AstAR1-Gal4 [21] N/A Drosophila AstA-RNAi VDRC 14398 Drosophila AstAR1-RNAi VDRC 39222 Drosophila AstAR1-RNAi VDRC 48495 Drosophila ptth-RNAi VDRC 102043 Drosophila UAS-dicer2 VDRC 60008 Drosophila UAS-dicer2 VDRC 60009 Drosophila op-LexA-GFP BDSC 58754 Drosophila UAS-RFP BDSC 58754 Drosophila CoinFLP-LexA::GAD BDSC 58753 Drosophila UAS-Kir2.1 BDSC 6595 (Continued on next page) Current Biology 29, 1–10.e1–e4, March 4, 2019 e1 ..
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